ABSTRACT
The antiviral effect of isoprinosine on simian rotavirus (SA-11) replication was studied using MA-104 cell cultures from Rhesus monkey fetal kidney. Isoprinosine (N,N-dimethylamino-2-propanol-p-acetamidobenzoate in association with inosine) added after viral infection (therapeutic test) inhibited viral replication by more than 90%. In these experiments, the drug was added to the medium and replaced daily at concentrations varying from 62.5 microng/ml to l mg/ml. Viral inhibition activity was dependent on drug concentration. No antiviral effect was observed when isoprinosine was tested without replacement (200-500 microng/ml). When isoprinosine (l mg/ml) was added to cell cultures only before viral infection (prophylactic test), inhibition of viral replication occurred but was less than 90%. Inhibition by less than 90% is not considered to be significant in this type of test. Isoprinosine inhibited synthesis of both viral antigen (protein) and viral double-stranded nucleic acid, as monitored by immunofluorescence and acridine orange staining, respectively. Inhibiton of synthesis of viral macromolecules increased with drug concentration
Subject(s)
In Vitro Techniques , Inosine Pranobex/pharmacology , Virus Replication , Rotavirus/physiology , Acridine Orange/pharmacology , Cells, Cultured , Culture Media , Cytopathogenic Effect, Viral , Microscopy, FluorescenceABSTRACT
A acao antiviral primaria de substancias naturais: ajmalina, extrato aquoso de Zoanthidae, extrato fluido de pau-d'-arco e fracoes extraidas com metanol e com diclorometano de Luidia senegalenns; substancias sinteticas: isoniazida, cloridrato de N1-(3-clorofenil)-N4-(3aminopropil) piperazina e N1-(3-clorofenil)-N4-(3-acetamidapropil) piperazina e a substancia semi-sintetica: usnato de isoniazida foi testada em celulas cultivadas em microplacas descartaveis. A atividade foi determinada pela inibicao dos efeitos citopaticos dos virus polio tipo 1, adeno tipo 5, herpes simples tipo 1 e virus da estomatite vesicular Alagoas, VSA, apos adicao de diferentes concentracooes das substancias.Antes de proceder a avaliacao da atividade antiviral, as substancias foram testadas quanto a toxicidade para as celulas utilizadas. Este metodo mostrou ser simples, sensivel, economico, de rapida execucao e permitiu detectar a acao de admalina e N1(3-clorofenil)-N4-(3-acetamidapropil) piperazina sobre VSA